Vacuolar protein sorting-associated protein 4A (EC 3.6.4.6) (Protein SKD2) (VPS4-1) (hVPS4)
1_MTTST 6_ LQKAI 11_ DLVTK 16_ ATEED 21_ KAKNY 26_ EEALR 31_ LYQHA 36_ VEYFL 41_ HAIKY 46_ EAHSD 51_ KAKES 56_ IRAKC 61_ VQYLD 66_ RAEKL 71_ KDYLR 76_ SKEKH 81_ GKKPV 86_ KENQS 91_ EGKGS 96_ DSDSE 101_ GDNPE 106_ KKKLQ 111_ EQLMG 116_ AVVME 121_ KPNIR 126_ WNDVA 131_ GLEGA 136_ KEALK 141_ EAVIL 146_ PIKFP 151_ HLFTG 156_ KRTPW 161_ RGILL 166_ FGPPG 171_ TGKSY 176_ LAKAV 181_ ATEAN 186_ NSTFF 191_ SVSSS 196_ DLMSK 201_ WLGES 206_ EKLVK 211_ NLFEL 216_ ARQHK 221_ PSIIF 226_ IDEVD 231_ SLCGS 236_ RNENE 241_ SEAAR 246_ RIKTE 251_ FLVQM 256_ QGVGN 261_ NNDGT 266_ LVLGA 271_ TNIPW 276_ VLDSA 281_ IRRRF 286_ EKRIY 291_ IPLPE 296_ EAARA 301_ QMFRL 306_ HLGST 311_ PHNLT 316_ DANIH 321_ ELARK 326_ TEGYS 331_ GADIS 336_ IIVRD 341_ SLMQP 346_ VRKVQ 351_ SATHF 356_ KKVCG 361_ PSRTN 366_ PSMMI 371_ DDLLT 376_ PCSPG 381_ DPGAM 386_ EMTWM 391_ DVPGD 396_ KLLEP 401_ VVCMS 406_ DMLRS 411_ LATTR 416_ PTVNA 421_ DDLLK 426_ VKKFS 431_EDFGQ
1: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. It is required for proper accomplishment of various processes including the regulation of endosome size, primary cilium organization, mitotic spindle organization, chromosome segregation, and nuclear envelope sealing and spindle disassembly during anaphase (PubMed:33186545). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Critical for normal erythroblast cytokinesis and correct erythropoiesis (PubMed:33186543)
2: (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses)