7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC 1.14.14.139) (7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase) (CYPVIIIB1) (Cytochrome P450 8B1) (Sterol 12-alpha-hydroxylase)
1_MVLWG 6_ PVLGA 11_ LLVVI 16_ AGYLC 21_ LPGML 26_ RQRRP 31_ WEPPL 36_ DKGTV 41_ PWLGH 46_ AMAFR 51_ KNMFE 56_ FLKRM 61_ RTKHG 66_ DVFTV 71_ QLGGQ 76_ YFTFV 81_ MDPLS 86_ FGSIL 91_ KDTQR 96_ KLDFG 101_ QYAKK 106_ LVLKV 111_ FGYRS 116_ VQGDH 121_ EMIHS 126_ ASTKH 131_ LRGDG 136_ LKDLN 141_ ETMLD 146_ SLSFV 151_ MLTSK 156_ GWSLD 161_ ASCWH 166_ EDSLF 171_ RFCYY 176_ ILFTA 181_ GYLSL 186_ FGYTK 191_ DKEQD 196_ LLQAG 201_ ELFME 206_ FRKFD 211_ LLFPR 216_ FVYSL 221_ LWPRE 226_ WLEVG 231_ RLQRL 236_ FHKML 241_ SVSHS 246_ QEKEG 251_ ISNWL 256_ GNMLQ 261_ FLREQ 266_ GVPSA 271_ MQDKF 276_ NFMML 281_ WASQG 286_ NTGPT 291_ SFWAL 296_ LYLLK 301_ HPEAI 306_ RAVRE 311_ EATQV 316_ LGEAR 321_ LETKQ 326_ SFAFK 331_ LGALQ 336_ HTPVL 341_ DSVVE 346_ ETLRL 351_ RAAPT 356_ LLRLV 361_ HEDYT 366_ LKMSS 371_ GQEYL 376_ FRHGD 381_ ILALF 386_ PYLSV 391_ HMDPD 396_ IHPEP 401_ TVFKY 406_ DRFLN 411_ PNGSR 416_ KVDFF 421_ KTGKK 426_ IHHYT 431_ MPWGS 436_ GVSIC 441_ PGRFF 446_ ALSEV 451_ KLFIL 456_ LMVTH 461_ FDLEL 466_ VDPDT 471_ PLPHV 476_ DPQRW 481_ GFGTM 486_ QPSHD 491_ VRFRY 496_RLHPT
1: A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis (PubMed:10051404). Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase) (By similarity)