RNA-binding protein Nova-2 (Astrocytic NOVA1-like RNA-binding protein) (Neuro-oncological ventral antigen 2)
1_MEPEA 6_ PDSRK 11_ RPLET 16_ PPEVV 21_ CTKRS 26_ NTGEE 31_ GEYFL 36_ KVLIP 41_ SYAAG 46_ SIIGK 51_ GGQTI 56_ VQLQK 61_ ETGAT 66_ IKLSK 71_ SKDFY 76_ PGTTE 81_ RVCLV 86_ QGTAE 91_ ALNAV 96_ HSFIA 101_ EKVRE 106_ IPQAM 111_ TKPEV 116_ VNILQ 121_ PQTTM 126_ NPDRA 131_ KQAKL 136_ IVPNS 141_ TAGLI 146_ IGKGG 151_ ATVKA 156_ VMEQS 161_ GAWVQ 166_ LSQKP 171_ EGINL 176_ QERVV 181_ TVSGE 186_ PEQVH 191_ KAVSA 196_ IVQKV 201_ QEDPQ 206_ SSSCL 211_ NISYA 216_ NVAGP 221_ VANSN 226_ PTGSP 231_ YASPA 236_ DVLPA 241_ AAAAS 246_ AAAAS 251_ GLLGP 256_ AGLAG 261_ VGAFP 266_ AALPA 271_ FSGTD 276_ LLAIS 281_ TALNT 286_ LASYG 291_ YNTNS 296_ LGLGL 301_ NSAAA 306_ SGVLA 311_ AVAAG 316_ ANPAA 321_ AAAAN 326_ LLASY 331_ AGEAG 336_ AGPAG 341_ GAAPP 346_ PPPPP 351_ GALGS 356_ FALAA 361_ AANGY 366_ LGAGA 371_ GGGAG 376_ GGGGP 381_ LVAAA 386_ AAAGA 391_ AGGFL 396_ TAEKL 401_ AAESA 406_ KELVE 411_ IAVPE 416_ NLVGA 421_ ILGKG 426_ GKTLV 431_ EYQEL 436_ TGARI 441_ QISKK 446_ GEFLP 451_ GTRNR 456_ RVTIT 461_ GSPAA 466_ TQAAQ 471_ YLISQ 476_ RVTYE 481_ QGVRA 486_SNPQK
1: Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion (PubMed:32197073). It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed:10811881). Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Uniquely regulates alternative splicing events of a series of axon guidance related genes during cortical development, being essential for central nervous system development by regulating neural networks wiring. Regulates differentially alternative splicing on the same transcripts expressed in different neurons. This includes functional differences in transcripts expressed in cortical and cerebellar excitatory versus inhibitory neurons where is required for, respectively, development of laminar structure and motor coordination and synapse formation. Also the regulation the regulation of intron retention can sequester the trans-acting splicing factor PTBP2, acting as a variable cis-acting scaffolding platform for PTBP2 across various natural conditions (By similarity)