Histone lysine demethylase PHF8 (EC 1.14.11.27) (EC 1.14.11.65) (PHD finger protein 8) ([histone H3]-dimethyl-L-lysine(36) demethylase PHF8) ([histone H3]-dimethyl-L-lysine(9) demethylase PHF8)
1_MNRSR 6_ AIVQR 11_ GRVLP 16_ PPAPL 21_ DTTNL 26_ AGRRT 31_ LQGRA 36_ KMASV 41_ PVYCL 46_ CRLPY 51_ DVTRF 56_ MIECD 61_ MCQDW 66_ FHGSC 71_ VGVEE 76_ EKAAD 81_ IDLYH 86_ CPNCE 91_ VLHGP 96_ SIMKK 101_ RRGSS 106_ KGHDT 111_ HKGKP 116_ VKTGS 121_ PTFVR 126_ ELRSR 131_ TFDSS 136_ DEVIL 141_ KPTGN 146_ QLTVE 151_ FLEEN 156_ SFSVP 161_ ILVLK 166_ KDGLG 171_ MTLPS 176_ PSFTV 181_ RDVEH 186_ YVGSD 191_ KEIDV 196_ IDVTR 201_ QADCK 206_ MKLGD 211_ FVKYY 216_ YSGKR 221_ EKVLN 226_ VISLE 231_ FSDTR 236_ LSNLV 241_ ETPKI 246_ VRKLS 251_ WVENL 256_ WPEEC 261_ VFERP 266_ NVQKY 271_ CLMSV 276_ RDSYT 281_ DFHID 286_ FGGTS 291_ VWYHV 296_ LKGEK 301_ IFYLI 306_ RPTNA 311_ NLTLF 316_ ECWSS 321_ SSNQN 326_ EMFFG 331_ DQVDK 336_ CYKCS 341_ VKQGQ 346_ TLFIP 351_ TGWIH 356_ AVLTP 361_ VDCLA 366_ FGGNF 371_ LHSLN 376_ IEMQL 381_ KAYEI 386_ EKRLS 391_ TADLF 396_ RFPNF 401_ ETICW 406_ YVGKH 411_ ILDIF 416_ RGLRE 421_ NRRHP 426_ ASYLV 431_ HGGKA 436_ LNLAF 441_ RAWTR 446_ KEALP 451_ DHEDE 456_ IPETV 461_ RTVQL 466_ IKDLA 471_ REIRL 476_ VEDIF 481_ QQNVG 486_ KTSNI 491_ FGLQR 496_ IFPAG 501_ SIPLT 506_ RPAHS 511_ TSVSM 516_ SRLSL 521_ PSKNG 526_ SKKKG 531_ LKPKE 536_ LFKKA 541_ ERKGK 546_ ESSAL 551_ GPAGQ 556_ LSYNL 561_ MDTYS 566_ HQALK 571_ TGSFQ 576_ KAKFN 581_ ITGAC 586_ LNDSD 591_ DDSPD 596_ LDLDG 601_ NESPL 606_ ALLMS 611_ NGSTK 616_ RVKSL 621_ SKSRR 626_ TKIAK 631_ KVDKA 636_ RLMAE 641_ QVMED 646_ EFDLD 651_ SDDEL 656_ QIDER 661_ LGKEK 666_ ATLII 671_ RPKFP 676_ RKLPR 681_ AKPCS 686_ DPNRV 691_ REPGE 696_ VEFDI 701_ EEDYT 706_ TDEDM 711_ VEGVE 716_ GKLGN 721_ GSGAG 726_ GILDL 731_ LKASR 736_ QVGGP 741_ DYAAL 746_ TEAPA 751_ SPSTQ 756_ EAIQG 761_ MLCMA 766_ NLQSS 771_ SSSPA 776_ TSSLQ 781_ AWWTG 786_ GQDRS 791_ SGSSS 796_ SGLGT 801_ VSNSP 806_ ASQRT 811_ PGKRP 816_ IKRPA 821_ YWRTE 826_ SEEEE 831_ ENASL 836_ DEQDS 841_ LGACF 846_ KDAEY 851_ IYPSL 856_ ESDDD 861_ DPALK 866_ SRPKK 871_ KKNSD 876_ DAPWS 881_ PKARV 886_ TPTLP 891_ KQDRP 896_ VREGT 901_ RVASI 906_ ETGLA 911_ AAAAK 916_ LAQQE 921_ LQKAQ 926_ KKKYI 931_ KKKPL 936_ LKEVE 941_ QPRPQ 946_ DSNLS 951_ LTVPA 956_ PTVAA 961_ TPQLV 966_ TSSSP 971_ LPPPE 976_ PKQEA 981_ LSGSL 986_ ADHEY 991_ TARPN 996_ AFGMA 1001_ QANRS 1006_ TTPMA 1011_ PGVFL 1016_ TQRRP 1021_ SVGSQ 1026_ SNQAG 1031_ QGKRP 1036_ KKGLA 1041_ TAKQR 1046_ LGRIL 1051_KIHRN
1: Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. Positively modulates transcription of histone demethylase KDM5C, acting synergistically with transcription factor ARX; synergy may be related to enrichment of histone H3K4me3 in regulatory elements