Chromodomain Y-like protein (CDY-like) (Crotonyl-CoA hydratase) (EC 4.2.1.-)
1_MTFQA 6_ SHRSA 11_ WGKSR 16_ KKNWQ 21_ YEGPT 26_ QKLFL 31_ KRNNV 36_ SAPDG 41_ PSDPS 46_ ISVSS 51_ EQSGA 56_ QQPPA 61_ LQVER 66_ IVDKR 71_ KNKKG 76_ KTEYL 81_ VRWKG 86_ YDSED 91_ DTWEP 96_ EQHLV 101_ NCEEY 106_ IHDFN 111_ RRHTE 116_ KQKES 121_ TLTRT 126_ NRTSP 131_ NNARK 136_ QISRS 141_ TNSNF 146_ SKTSP 151_ KALVI 156_ GKDHE 161_ SKNSQ 166_ LFAAS 171_ QKFRK 176_ NTAPS 181_ LSSRK 186_ NMDLA 191_ KSGIK 196_ ILVPK 201_ SPVKS 206_ RTAVD 211_ GFQSE 216_ SPEKL 221_ DPVEQ 226_ GQEDT 231_ VAPEV 236_ AAEKP 241_ VGALL 246_ GPGAE 251_ RARMG 256_ SRPRI 261_ HPLVP 266_ QVPGP 271_ VTAAM 276_ ATGLA 281_ VNGKG 286_ TSPFM 291_ DALTA 296_ NGTTN 301_ IQTSV 306_ TGVTA 311_ SKRKF 316_ IDDRR 321_ DQPFD 326_ KRLRF 331_ SVRQT 336_ ESAYR 341_ YRDIV 346_ VRKQD 351_ GFTHI 356_ LLSTK 361_ SSENN 366_ SLNPE 371_ VMREV 376_ QSALS 381_ TAAAD 386_ DSKLV 391_ LLSAV 396_ GSVFC 401_ CGLDF 406_ IYFIR 411_ RLTDD 416_ RKRES 421_ TKMAE 426_ AIRNF 431_ VNTFI 436_ QFKKP 441_ IIVAV 446_ NGPAI 451_ GLGAS 456_ ILPLC 461_ DVVWA 466_ NEKAW 471_ FQTPY 476_ TTFGQ 481_ SPDGC 486_ STVMF 491_ PKIMG 496_ GASAN 501_ EMLLS 506_ GRKLT 511_ AQEAC 516_ GKGLV 521_ SQVFW 526_ PGTFT 531_ QEVMV 536_ RIKEL 541_ ASCNP 546_ VVLEE 551_ SKALV 556_ RCNMK 561_ MELEQ 566_ ANERE 571_ CEVLK 576_ KIWGS 581_ AQGMD 586_ SMLKY 591_LQRKI
1: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed:19808672, PubMed:28402439). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed:28402439). Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (PubMed:22009739). Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (PubMed:19061646). Involved in X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (By similarity). Promotes EZH2 accumulation and H3K27me3 methylation at DNA double strand breaks (DSBs), thereby facilitating transcriptional repression at sites of DNA damage and homology-directed repair of DSBs (PubMed:29177481). Required for neuronal migration during brain development by repressing expression of RHOA (By similarity). By repressing the expression of SCN8A, contributes to the inhibition of intrinsic neuronal excitability and epileptogenesis (By similarity). In addition to acting as a chromatin reader, acts as a hydro-lyase (PubMed:28803779). Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (PubMed:28803779). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (By similarity). By regulating histone crotonylation and trimethylation of H3K27, may be involved in stress-induced depression-like behaviors, possibly by regulating VGF expression (By similarity)
2: Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the presence of a N-terminal extension that inactivates the chromo domain (PubMed:19808672)
3: Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the absence of the chromo domain (PubMed:19808672). Acts as a negative regulator of isoform 2 by displacing isoform 2 from chromatin