Sialidase-2 (EC 3.2.1.18) (Cytosolic sialidase) (N-acetyl-alpha-neuraminidase 2)
1_MASLP 6_ VLQKE 11_ SVFQS 16_ GAHAY 21_ RIPAL 26_ LYLPG 31_ QQSLL 36_ AFAEQ 41_ RASKK 46_ DEHAE 51_ LIVLR 56_ RGDYD 61_ APTHQ 66_ VQWQA 71_ QEVVA 76_ QARLD 81_ GHRSM 86_ NPCPL 91_ YDAQT 96_ GTLFL 101_ FFIAI 106_ PGQVT 111_ EQQQL 116_ QTRAN 121_ VTRLC 126_ QVTST 131_ DHGRT 136_ WSSPR 141_ DLTDA 146_ AIGPA 151_ YREWS 156_ TFAVG 161_ PGHCL 166_ QLHDR 171_ ARSLV 176_ VPAYA 181_ YRKLH 186_ PIQRP 191_ IPSAF 196_ CFLSH 201_ DHGRT 206_ WARGH 211_ FVAQD 216_ TLECQ 221_ VAEVE 226_ TGEQR 231_ VVTLN 236_ ARSHL 241_ RARVQ 246_ AQSTN 251_ DGLDF 256_ QESQL 261_ VKKLV 266_ EPPPQ 271_ GCQGS 276_ VISFP 281_ SPRSG 286_ PGSPA 291_ QWLLY 296_ THPTH 301_ SWQRA 306_ DLGAY 311_ LNPRP 316_ PAPEA 321_ WSEPV 326_ LLAKG 331_ SCAYS 336_ DLQSM 341_ GTGPD 346_ GSPLF 351_ GCLYE 356_ ANDYE 361_ EIVFL 366_ MFTLK 371_QAFPA
1: Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides (PubMed:14613940, PubMed:22228546). Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha-(2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)-sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed:14613940). Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha-(2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha-(2->8)-linked Neu5Ac residues (PubMed:14613940)