WASH complex subunit 2C (Vaccinia virus penetration factor) (VPEF)
1_MMNRT 6_ TPDQE 11_ LVPAS 16_ EPVWE 21_ RPWSV 26_ EEIRR 31_ SSQSW 36_ SLAAD 41_ AGLLQ 46_ FLQEF 51_ SQQTI 56_ SRTHE 61_ IKKQV 66_ DGLIR 71_ ETKAT 76_ DCRLH 81_ NVFND 86_ FLMLS 91_ NTQFI 96_ ENRVY 101_ DEEVE 106_ EPVLK 111_ AEAEK 116_ TEQEK 121_ TREQK 126_ EVDLI 131_ PKVQE 136_ AVNYG 141_ LQVLD 146_ SAFEQ 151_ LDIKA 156_ GNSDS 161_ EEDDA 166_ NGRVE 171_ LILEP 176_ KDLYI 181_ DRPLP 186_ YLIGS 191_ KLFME 196_ QEDVG 201_ LGELS 206_ SEEGS 211_ VGSDR 216_ GSIVD 221_ TEEEK 226_ EEEES 231_ DEDFA 236_ HHSDN 241_ EQNQH 246_ TTQMS 251_ DEEED 256_ DDGCD 261_ LFADS 266_ EKEEE 271_ DIEDI 276_ EENTR 281_ PKRSR 286_ PTSFA 291_ DELAA 296_ RIKGD 301_ AMGRV 306_ DEEPT 311_ TLPSG 316_ EAKPR 321_ KTLKE 326_ KKERR 331_ TPSDD 336_ EEDNL 341_ FAPPK 346_ LTDED 351_ FSPFG 356_ SGGGL 361_ FSGGK 366_ GLFDD 371_ EDEES 376_ DLFTE 381_ ASQDR 386_ QAGAS 391_ VKEES 396_ SSSKP 401_ GKKIP 406_ AGAVS 411_ VFLGD 416_ TDVFG 421_ AASVP 426_ SLKEP 431_ QKPEQ 436_ PTPRK 441_ SPYGP 446_ PPTGL 451_ FDDDD 456_ GDDDD 461_ DFFSA 466_ PHSKP 471_ SKTRK 476_ VQSTA 481_ DIFGD 486_ EEGDL 491_ FKEKA 496_ VASPE 501_ ATVSQ 506_ TDENK 511_ ARAEK 516_ KVTLS 521_ YSKNL 526_ KPSSE 531_ TKTQK 536_ GLFSD 541_ EEDSE 546_ DLFSS 551_ QSASN 556_ LKGAS 561_ LLPGK 566_ LPTSV 571_ SLFDD 576_ EDEED 581_ NLFGG 586_ TAAKK 591_ QTLSL 596_ QAQRE 601_ EKAKA 606_ SELSK 611_ KKASA 616_ LLFSS 621_ DEEDQ 626_ WNIPA 631_ SQTHL 636_ ASDSR 641_ SKGEP 646_ RDSGT 651_ LQSQE 656_ AKAVK 661_ KTSLF 666_ EEDKE 671_ DDLFA 676_ IAKDS 681_ QKKTQ 686_ RVSLL 691_ FEDDV 696_ DSGGS 701_ LFGSP 706_ PTSVP 711_ PATKK 716_ KETVS 721_ EAPPL 726_ LFSDE 731_ EEKEA 736_ QLGVK 741_ SVDKK 746_ VESAK 751_ ESLKF 756_ GRTDV 761_ AESEK 766_ EGLLT 771_ RSAQE 776_ TVKHS 781_ DLFSS 786_ SSPWD 791_ KGTKP 796_ RTKTV 801_ LSLFD 806_ EEEDK 811_ MEDQN 816_ IIQAP 821_ QKEVG 826_ KGCDP 831_ DAHPK 836_ STGVF 841_ QDEEL 846_ LFSHK 851_ LQKDN 856_ DPDVD 861_ LFAGT 866_ KKTKL 871_ LEPSV 876_ GSLFG 881_ DDEDD 886_ DLFSS 891_ AKSQP 896_ LVQEK 901_ KRVVK 906_ KDHSV 911_ NSFKN 916_ QKHPE 921_ SIQGS 926_ KEKGI 931_ WKPET 936_ PQANL 941_ AINPA 946_ ALLPT 951_ AASQI 956_ SEVKP 961_ VLPEL 966_ AFPSS 971_ EHRRS 976_ HGLES 981_ VPVLP 986_ GSGEA 991_ GVSFD 996_ LPAQA 1001_ DTLHS 1006_ ANKSR 1011_ VKMRG 1016_ KRRPQ 1021_ TRAAR 1026_ RLAAQ 1031_ ESSEA 1036_ EDMSV 1041_ PRGPI 1046_ AQWAD 1051_ GAISP 1056_ NGHRP 1061_ QLRAA 1066_ SGEDS 1071_ TEEAL 1076_ AAAAA 1081_ PWEGG 1086_ PVPGV 1091_ DTSPF 1096_ AKSLG 1101_ HSRGE 1106_ ADLFD 1111_ SGDIF 1116_ STGTG 1121_ SQSVE 1126_ RTKPK 1131_ AKIAE 1136_ NPANP 1141_ PVGGK 1146_ AKSPM 1151_ FPALG 1156_ EASSD 1161_ DDLFQ 1166_ SAKPK 1171_ PAKKT 1176_ NPFPL 1181_ LEDED 1186_ DLFTD 1191_ QKVKK 1196_ NETKS 1201_ SSQQD 1206_ VILTT 1211_ QDIFE 1216_ DDIFA 1221_ TEAIK 1226_ PSQKT 1231_ REKEK 1236_ TLESN 1241_ LFDDN 1246_ IDIFA 1251_ DLTVK 1256_ PKEKS 1261_ KKKVE 1266_ AKSIF 1271_ DDDMD 1276_ DIFST 1281_ GIQAK 1286_ TTKPK 1291_ SRSAQ 1296_ AAPEP 1301_ RFEHK 1306_ VSNIF 1311_DDPLN
1: Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission (PubMed:19922874, PubMed:20498093, PubMed:22513087, PubMed:23331060). Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35 (PubMed:24980502). Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L (PubMed:25355947, PubMed:28892079)
2: (Microbial infection) Plays a role in fluid-phase endocytosis, a process exploited by vaccinia intracellular mature virus (IMV) to enter cells. As a result, may facilitate the penetration of IMV into cells