WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2) (WIPI49-like protein 2)
1_MNLAS 6_ QSGEA 11_ GAGQL 16_ LFANF 21_ NQDNT 26_ EVKGA 31_ SRAAG 36_ LGRRA 41_ VVWSL 46_ AVGSK 51_ SGYKF 56_ FSLSS 61_ VDKLE 66_ QIYEC 71_ TDTED 76_ VCIVE 81_ RLFSS 86_ SLVAI 91_ VSLKA 96_ PRKLK 101_ VCHFK 106_ KGTEI 111_ CNYSY 116_ SNTIL 121_ AVKLN 126_ RQRLI 131_ VCLEE 136_ SLYIH 141_ NIRDM 146_ KVLHT 151_ IRETP 156_ PNPAG 161_ LCALS 166_ INNDN 171_ CYLAY 176_ PGSAT 181_ IGEVQ 186_ VFDTI 191_ NLRAA 196_ NMIPA 201_ HDSPL 206_ AALAF 211_ DASGT 216_ KLATA 221_ SEKGT 226_ VIRVF 231_ SIPEG 236_ QKLFE 241_ FRRGV 246_ KRCVS 251_ ICSLA 256_ FSMDG 261_ MFLSA 266_ SSNTE 271_ TVHIF 276_ KLETV 281_ KEKPP 286_ EEPTT 291_ WTGYF 296_ GKVLM 301_ ASTSY 306_ LPSQV 311_ TEMFN 316_ QGRAF 321_ ATVRL 326_ PFCGH 331_ KNICS 336_ LATIQ 341_ KIPRL 346_ LVGAA 351_ DGYLY 356_ MYNLD 361_ PQEGG 366_ ECALM 371_ KQHRL 376_ DGSLE 381_ TTNEI 386_ LDSAS 391_ HDCPL 396_ VTQTY 401_ GAAAG 406_ KGTYV 411_ PSSPT 416_ RLAYT 421_ DDLGA 426_ VGGAC 431_ LEDEA 436_ SALRL 441_ DEDSE 446_HPPMI
1: Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:20505359, PubMed:28561066). Involved in an early step of the formation of preautophagosomal structures (PubMed:20505359, PubMed:28561066). Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases (PubMed:28561066). Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P (PubMed:28890335). Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed:20505359, PubMed:28561066)
2: Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella