Tubulin monoglycylase TTLL3 (EC 6.3.2.-) (HOTTL) (Tubulin--tyrosine ligase-like protein 3)
1_MNRLR 6_ NAKIY 11_ VERAV 16_ KQKKI 21_ FTIQG 26_ CYPVI 31_ RCLLR 36_ RRGWV 41_ EKKMV 46_ HRSGP 51_ TLLPP 56_ QKDLD 61_ SSAMG 66_ DSDTT 71_ EDEDE 76_ DEDEE 81_ FQPSQ 86_ LFDFD 91_ DLLKF 96_ DDLDG 101_ THALM 106_ VGLCL 111_ NLRNL 116_ PWFDE 121_ VDANS 126_ FFPRC 131_ YCLGA 136_ EDDKK 141_ AFIED 146_ FWLTA 151_ ARNVL 156_ KLVVK 161_ SEWKS 166_ YPIQA 171_ VEEEA 176_ SGDKQ 181_ PKKQE 186_ KNPVL 191_ VSPEF 196_ VDEAL 201_ CACEE 206_ YLSNL 211_ AHMDI 216_ DKDLE 221_ APLYL 226_ TPEGW 231_ SLFLQ 236_ RYYQV 241_ VHEGA 246_ ELRHL 251_ DTQVQ 256_ RCEDI 261_ LQQLQ 266_ AVVPQ 271_ IDMEG 276_ DRNIW 281_ IVKPG 286_ AKSRG 291_ RGIMC 296_ MDHLE 301_ EMLKL 306_ VNGNP 311_ VVMKD 316_ GKWVV 321_ QKYIE 326_ RPLLI 331_ FGTKF 336_ DLRQW 341_ FLVTD 346_ WNPLT 351_ VWFYR 356_ DSYIR 361_ FSTQP 366_ FSLKN 371_ LDNSV 376_ HLCNN 381_ SIQKH 386_ LENSC 391_ HRHPL 396_ LPPDN 401_ MWSSQ 406_ RFQAH 411_ LQEMG 416_ APNAW 421_ STIIV 426_ PGMKD 431_ AVIHA 436_ LQTSQ 441_ DTVQC 446_ RKASF 451_ ELYGA 456_ DFVFG 461_ EDFQP 466_ WLIEI 471_ NASPT 476_ MAPST 481_ AVTAR 486_ LCAGV 491_ QADTL 496_ RVVID 501_ RMLDR 506_ NCDTG 511_ AFELI 516_ YKQPA 521_ VEVPQ 526_ YVGIR 531_ LLVEG 536_ FTIKK 541_ PMAMC 546_ HRRMG 551_ VRPAV 556_ PLLTQ 561_ RGSGE 566_ ARHHF 571_ PSLHT 576_ KAQLP 581_ SPHVL 586_ RHQGQ 591_ VLRRQ 596_ HSKLV 601_ GTKAL 606_ STTGK 611_ ALRTL 616_ PTAKV 621_ FISLP 626_ PNLDF 631_ KVAPS 636_ ILKPR 641_ KAPAL 646_ LCLRG 651_ PQLEV 656_ PCCLC 661_ PLKSE 666_ QFLAP 671_ VGRSR 676_ PKANS 681_ RPDCD 686_ KPRAE 691_ ACPMK 696_ RLSPL 701_ KPLPL 706_ VGTFQ 711_ RRRGL 716_ GDMKL 721_ GKPLL 726_ RFPTA 731_ LVLDP 736_ TPNKK 741_ KQVKY 746_ LGLDS 751_ IAVGG 756_ SRVDG 761_ ARPCT 766_PGSTA
1: Monoglycylase which modifies alpha- and beta-tubulin, adding a single glycine on the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of tubulin. Not involved in elongation step of the polyglycylation reaction (By similarity). Preferentially glycylates a beta-tail peptide over the alpha-tail, although shifts its preference toward alpha-tail as beta-tail glutamylation increases (By similarity). Competes with polyglutamylases for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions (By similarity). Together with TTLL8, mediates microtubule glycylation of primary and motile cilia, which is essential for their stability and maintenance (By similarity). Involved in microtubule glycylation of primary cilia in colon which controls cell proliferation of epithelial cells and plays an essential role in colon cancer development (PubMed:25180231). Together with TTLL8, glycylates sperm flagella which regulates axonemal dynein motor activity, thereby controlling flagellar beat, directional sperm swimming and male fertility (By similarity)