Dolichyl-phosphate beta-glucosyltransferase (DolP-glucosyltransferase) (EC 2.4.1.117) (Asparagine-linked glycosylation protein 5 homolog)
1_MAPLL 6_ LQLAV 11_ LGAAL 16_ AAAAL 21_ VLISI 26_ VAFTT 31_ ATKMP 36_ ALHRH 41_ EEEKF 46_ FLNAK 51_ GQKET 56_ LPSIW 61_ DSPTK 66_ QLSVV 71_ VPSYN 76_ EEKRL 81_ PVMMD 86_ EALSY 91_ LEKRQ 96_ KRDPA 101_ FTYEV 106_ IVVDD 111_ GSKDQ 116_ TSKVA 121_ FKYCQ 126_ KYGSD 131_ KVRVI 136_ TLVKN 141_ RGKGG 146_ AIRMG 151_ IFSSR 156_ GEKIL 161_ MADAD 166_ GATKF 171_ PDVEK 176_ LEKGL 181_ NDLQP 186_ WPNQM 191_ AIACG 196_ SRAHL 201_ EKESI 206_ AQRSY 211_ FRTLL 216_ MYGFH 221_ FLVWF 226_ LCVKG 231_ IRDTQ 236_ CGFKL 241_ FTREA 246_ ASRTF 251_ SSLHV 256_ ERWAF 261_ DVELL 266_ YIAQF 271_ FKIPI 276_ AEIAV 281_ NWTEI 286_ EGSKL 291_ VPFWS 296_ WLQMG 301_ KDLLF 306_ IRLRY 311_ LTGAW 316_RLEQT
1: Dolichyl-phosphate beta-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Dolichyl-phosphate beta-glucosyltransferase produces dolichyl beta-D-glucosyl phosphate/Dol-P-Glc, the glucose donor substrate used sequentially by ALG6, ALG8 and ALG10 to add glucose residues on top of the Man(9)GlcNAc(2)-PP-Dol structure. These are the three last steps in the biosynthetic pathway of dolichol-linked oligosaccharides to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol. The enzyme is most probably active on the cytoplasmic side of the endoplasmic reticulum while its product Dol-P-Glc is the substrate for ALG6, ALG8 and ALG11 in the lumen of the endoplasmic reticulum