Cholesterol 24-hydroxylase (CH24H) (EC 1.14.14.25) (Cholesterol 24-monooxygenase) (Cholesterol 24S-hydroxylase) (Cytochrome P450 46A1)
1_MSPGL 6_ LLLGS 11_ AVLLA 16_ FGLCC 21_ TFVHR 26_ ARSRY 31_ EHIPG 36_ PPRPS 41_ FLLGH 46_ LPCFW 51_ KKDEV 56_ GGRVL 61_ QDVFL 66_ DWAKK 71_ YGPVV 76_ RVNVF 81_ HKTSV 86_ IVTSP 91_ ESVKK 96_ FLMST 101_ KYNKD 106_ SKMYR 111_ ALQTV 116_ FGERL 121_ FGQGL 126_ VSECN 131_ YERWH 136_ KQRRV 141_ IDLAF 146_ SRSSL 151_ VSLME 156_ TFNEK 161_ AEQLV 166_ EILEA 171_ KADGQ 176_ TPVSM 181_ QDMLT 186_ YTAMD 191_ ILAKA 196_ AFGME 201_ TSMLL 206_ GAQKP 211_ LSQAV 216_ KLMLE 221_ GITAS 226_ RNTLA 231_ KFLPG 236_ KRKQL 241_ REVRE 246_ SIRFL 251_ RQVGR 256_ DWVQR 261_ RREAL 266_ KRGEE 271_ VPADI 276_ LTQIL 281_ KAEEG 286_ AQDDE 291_ GLLDN 296_ FVTFF 301_ IAGHE 306_ TSANH 311_ LAFTV 316_ MELSR 321_ QPEIV 326_ ARLQA 331_ EVDEV 336_ IGSKR 341_ YLDFE 346_ DLGRL 351_ QYLSQ 356_ VLKES 361_ LRLYP 366_ PAWGT 371_ FRLLE 376_ EETLI 381_ DGVRV 386_ PGNTP 391_ LLFST 396_ YVMGR 401_ MDTYF 406_ EDPLT 411_ FNPDR 416_ FGPGA 421_ PKPRF 426_ TYFPF 431_ SLGHR 436_ SCIGQ 441_ QFAQM 446_ EVKVV 451_ MAKLL 456_ QRLEF 461_ RLVPG 466_ QRFGL 471_ QEQAT 476_ LKPLD 481_ PVLCT 486_ LRPRG 491_WQPAP
1: P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation (PubMed:10377398, PubMed:14640697, PubMed:18621681, PubMed:25017465). By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (By similarity). Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis (PubMed:12077124, PubMed:14640697, PubMed:28190002). Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain (PubMed:25017465). May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole (PubMed:20667828)